In this study， a TaqMan real-time PCR method was established for rapid detection of black truffle derived ingredients. Specific primers and probes were designed according to conserved sequence of ITS gene of Tuber indicum complex to test the target gene fragment in the samples， and species-specific detection， sensitivity detection and practical application detection were carried out. Results showed that this real-time PCR method had strong specificity， only showed specific amplification curves for black truffle genomic DNA， but there was no amplification curve for other edible fungi， animal and plant materials. The limit of detection was 1.0×10-3 ng/μL genomic DNA or 0.01% (mass fraction) per reaction. The feasibility of the method was further verified by detecting 120 commercial black truffles (fresh， frozen， dry and powder)， black truffle products (black truffle sauce and black truffle seasoning powder) and miscellaneous bacteria bags without black truffle components. The black truffle components were detected in 70 black truffles and 10 black truffle products. This method has the characteristics of strong specificity， high sensitivity， rapidity and high sufficiency， and is suitable for rapid identification of black truffles ingredients.