HPTLC Combines Pixel and Optical Density Method to Fast Quantitative EGCG in Tea Drinks
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(1.Tsui Xiangyuan Health Food (Zhongshan) Co., Ltd., Zhongshan 528414, Guangdong;2.China Institute of Standardization, Beijing 100191;3.School of Food Science, Jiangnan University, Wuxi 214122, Jiangsu;4.School of Food Science and Engineering, South China University of Technology, Guangzhou 510641;5.College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, Shanxi)

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    Abstract:

    This study by HPTLC analysis platform, use the pixels in a parallel analysis and optical density method, set up and compared two kinds of rapid detection of EGCG in tea drinks. With three kinds of tea drinks sold as representative samples, compare two methods of application performance, evaluate alternative instrument depend on the optical density of pixel analysis testing as tea drink. The results showed that the detection limits of HPTLC-pixel analysis method and HPTLC-optical density method were 6.8 mg/kg and 5 mg/kg, respectively, which were sufficient to quantify EGCG in tea beverages; Two methods of target in 200-1200 ng/band concentration within the scope of the linear correlation coefficient were small and reached more than 0.999. The recoveries of the two methods meet the requirements, the spiked recoveries of the three samples determinedby the HPTLC-pixel analytical method ranged from 102.4% to 110.8%, 102.3% to 118.8% and 100.3% to 113.5%, the spiked recoveries of the three samples determinedby the HPTLC-optical density method ranged from 103.5% to 110.8%, 95.3% to 111.2%, and 99.3% to 111.2%, respectively. Indicating that the two methods have good accuracy. The precision of the two methods is also similar, HPTLC-pixel analytical method RSD<6.7%, HPTLC-optical density method RSD<3.2%. In conclusion, in HPTLC detection technology, simple and easy-to-use pixel analysis can better replace expensive instrumented densitometric scanning, improving the cost-effectiveness and practicability of the method.

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  • Received:September 21,2022
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  • Online: November 22,2023
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