Evaluation of the Structure, in Vitro Antioxidant and Hypoglycemic Activity of Insoluble Dietary Fiber from Litchi Pomace
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(1.Sericultural & Agri-Food Research Institute Guangdong Academy of Agricultural Sciences,Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610;2.College of Food Sciences, South China Agricultural University, Guangzhou 510642;3.College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510631)

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    Abstract:

    In order to study the effects of different extraction methods on the physicochemical, structural and functional properties of insoluble dietary fiber (IDF) from litchi pomace, four methods of enzymatic hydrolysis, ultrasound, high-pressure hot water and high hydrostatic pressure were used to prepare IDF from litchi pomace, and the color, particle size, monosaccharide composition and structural properties of IDF from litchi pomace were analyzed by laser particle size meter, high performance liquid chromatography and fourier infrared spectroscopy, and the in vitro antioxidant and hypoglycaemic abilities of litchi pomace IDFs were investigated. The results showed that the average particle size of litchi pomace IDF extracted by the enzymatic method was the smallest [(11.88±1.19) μm]. The enzymatic and ultrasonic methods contained more rhamnose, galacturonic acid, glucose, galactose and arabinose. The structural properties showed no significant changes in the characteristic absorption peaks and crystal conformation of the cellulose polysaccharides for each fiber sample, while there were differences in the microstructure. The surface structure of each litchi pomace IDF was rough and loose, with many folds and gaps. Characteristically, the surface morphology of the litchi pomace IDF extracted by the ultrasonic method was puffy, with a high number of fibrous bundles present. The functional properties showed that the total phenolic content of litchi pomace IDF extracted by high-pressure hot water method was up to (13.23±0.32) mg GAE/g, with the most significant effect on the scavenging of DPPH and ABTS radicals. The litchi pomace IDF extracted by high-pressure hot water method and high hydrostatic pressure method showed the strongest α-glucosidase inhibitory activity. The glucose binding ability and the delayed glucose dialysis capacity of the ultrasonic extracted litchi pomace IDF were significantly higher than those of the other samples. In conclusion, litchi pomace IDF has potential antioxidant and hypoglycemic activities and can be a good resource for functional product.

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  • Received:October 22,2022
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  • Online: December 04,2023
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