Purification Technology of Peanut Red Procyanidins and Its Effect on Celiac Disease Cell Model
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(1.College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046;2.College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002;3.Longhu Laboratory, Zhengzhou 450046;4.International Joint Research Center of Animal Immunology, Henan Agricultural University, Zhengzhou 450046;5.Zhengzhou Key Laboratory of Nutrition and Health Food, Zhengzhou 450002)

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    Abstract:

    Objective: To optimize the purification process of AB-8 macroporous resin for peanut red procyanidins and to characterize and identify the components of the purified product, and to evaluate its inhibitory effect on cytotoxicity in celiac disease model. Methods: AB-8 macroporous resin was selected, and the purification conditions were optimized by static and dynamic adsorption-desorption indexes. The purified material was analyzed and identified by Ultraviolet/visible spectroscopy, Fourier transform infrared spectroscopy and High performance liquid chromatography-tandem mass spectrometry (UPLC-QTOF-MS/MS). The inhibitory effect of purified material on cytotoxicity was evaluated based on celiac disease cell model. Results: Purification conditions: The adsorption time was 180 min. The desorption solution was 120 mL 40% ethanol solution. The desorption time was 40 min. The desorption flow rate was 1.0 mg/mL. Ultraviolet/visible spectrum analysis showed that the purified substance belonged to proanthocyanidins. Fourier infrared analysis showed that protocyanin was the main structural unit of the purified product, which was consistent with the characteristic absorption peak trend of the standard product. UPLC-QTOF-MS/MS analysis showed that type A procyanidins were the main component of Pspc purified products. The results of cell test showed that the purified substance could significantly inhibit the toxicity of celiac disease model to some extent. This study can provide some theoretical reference for comprehensive development of peanut red resources.

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History
  • Received:December 25,2022
  • Online: January 23,2024
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