Objective: This study was conducted to investigate the effects of rice bran phenolic extracts (RBPE) supplementation on glucose metabolism and oxidative stress in high-fat diet induced mice， which could provide theoretical basis for revealing the effect of rice bran on glucose metabolism. Methods: After 1 week of acclimatization， mice were randomly divided into 4 groups: normal control group， high-fat diet group， low-dose RBPE group (200 mg/kg， HF+LP group) and high-dose RBPE group (400 mg/kg， HF+HP group). The experiment lasted for 16 weeks. The organ-body ratio， epididymal fat morphology， fasting serum glucose level， oral glucose tolerance and the levels of oxidative stress were measured. Results: RBPE dose-dependently decreased the organ-body ratio， fasting blood glucose and ameliorated the impaired glucose tolerance induced by high-fat diet. Compared with the high-fat diet group， organ-body ratio of the liver of HF+LP and HF+HP groups were decreased by 15.75% and 39.61%， respectively， the organ-body ratio of epididymal adipose tissue decreased by 23.03% and 39.83%， respectively， the organ-body ratio of renal peritoneal adipose tissue decreased by 43.68% and 56.83%， respectively， fasting blood glucose levels decreased by 14.06% and 20.21%， and the area under the glucose tolerance curve decreased by 19.06% and 23.81%， respectively. Further studies showed that low and high doses of RBPE can significantly reduce serum and liver tissue MDA content and increase SOD activity in high-fat diet induced mice. Compared with the high-fat diet group mice， serum and liver MDA content in HF+HP group mice were decreased by 48.93% and 38.68%， respectively， SOD activity increased by 80.44% and 67.05%， respectively； RBPE treatment could also increase antioxidant enzyme activities such as catalase (CAT) and glutathione peroxidase (GSH-px) and the content of antioxidant glutathione (GSH) in liver tissue of high-fat diet induced mice (P<0.05). Compared with the high-fat diet group mice， CAT， GSH-px activity and GSH content in HF+HP group mice were increased by 48.49%， 31.67% and 123.90%， respectively. Conclusion: RBPE could alleviate the impaired glucose metabolism induced by high-fat diet， which might be related to its inhibition effect on oxidative stress.