To study the antioxidant effect in vivo and the effect of alleviating visual fatigue of blueberry pulp， and analyze the correlation between the antioxidant function of blueberry pulp and the effect of improving visual fatigue. The protein content， superoxide dismutase (SOD) activity， catalase (CAT) activity and malondialdehyde (MDA) content of mice were determined by kit after 30 days of low， medium and high doses of blueberry pulp. A total of 40 volunteers aged 18-22 with visual fatigue symptoms were selected and randomly divided into control group and trial drinking group. The trial drinking group drank 2 bottles of blueberry pulp (100 mL/d) every day for consecutive 60 d. The results showed as follows: Compared with blank control group， the serum SOD activity of mice was significantly increased to 610.76 U/mg prot， while the serum MDA content was significantly decreased to 4.82 nmol/mg prot in the intake of 0.53 mL blueberry pulp (P<0.05)， and the intake of 0.21 mL blueberry pulp could significantly increase CAT activity in mice to 8.58 U/mg prot (P<0.05). The human body test showed that after drinking the blueberry juice for 60 d， the symptoms of visual fatigue and the duration of photopic vision were improved in the test group， and there was a significant difference in the male group (P<0.05). Result: Blueberry pulp has good antioxidant properties as well as good for relieving visual fatigue.