In this study， antifreeze crude peptide was obtained from crucian carp fish scales by trypsin enzyme digestion. And the formulation was designed by using {3， 3} simplex lattice method with antifreeze crude peptide， trehalose and alginate oligosaccharides as factors. The survival rate of Streptococcus pyogenes (S. pyogenes) after 24 h of freezing was used as the screening index for antifreeze activity to obtain the theoretical model formulation of the compound antifreeze. The optimum group of composite antifreeze (K1， K2) and the theoretical model formulation group (K3) were further selected for the frozen channel catfish(Ictalurus punctatus) myofibrillar protein test to verify the antifreeze effect of the composite antifreeze. The results showed that the survival rate of S. pyogenes in the antifreeze crude peptide group reached only 57.11%. The infrared spectra of the interaction with soy lecithin showed that the crude antifreeze peptide might interact with the hydrophilic head of phospholipids and water， which could help to stabilize the membrane structure. The survival rate of S. pyogenes reached more than 80% in the K1 and K2 groups， and as high as 92% in the K3 group. The hydrophobicity of the protein surface was significantly reduced (P< 0.05) and the protein solubility was significantly increased (P< 0.05) by the addition of K1， K2 and K3 complex antifreeze. The α-helix and β-fold content of K1 and K2 were significantly decreased (P< 0.05)； and the r-values of K2 and K3 were significantly decreased (P< 0.05). In summary， the theoretical model K3 is a good aquatic products composite antifreeze with high antifreeze activity， which can inhibit protein aggregation and maintain the stability of protein structure. This study provides a new idea for the efficient development of composite antifreeze， and provides a theoretical basis for the subsequent development of catfish pre-prepared vegetables frozen products.