Objective: To study the effects of fructose intake with different dosages on function indicators of liver and pancreas in rats for the healthy consumption of fructose. Methods: SD rats were fed a diet containing fructose at doses of 0% (CON)， 7.5% (LFD)， 12.75% (MFD) and 35.0% (HFD)， respectively， for 8 weeks. After anesthesia by carbon dioxide and execution by cervical dislocation， blood， liver， and pancreatic tissues were collected. The changes of liver and pancreatic function related indexes were detected and analyzed by immunobiochemical analysis and Western blot. Results: There was no significant difference in body weight and food intake among rats treated with different doses of fructose (P>0.05). There were no significant changes in blood lipids， liver and pancreas function indicators in LFD and MFD groups compared with CON group， while the hepatosomatic index (HSI) in the HFD group was significantly higher (P<0.05)， and the serum TC and TG contents were the highest. The serum SOD activity in HFD group was the lowest， and the GSH-Px level was significantly decreased (P<0.05)， while MDA level and AST/ ALT value were both significantly increased (P<0.05)， when compared with CON group. Moreover， In HFD group， the mRNA expression levels of HO-1 and GSH in liver tissue decreased significantly (P<0.05)， while the total expression of fat accumulation related pat protein increased significantly (P<0.05). In addition， the blood glucose and insulin levels in HFD group were significantly higher than those in CON， LFD and MFD groups (P<0.05)， and the islets were found shrinking. Furthermore， this study also found that the level of blood uric acid in HFD group was significantly higher than that in the CON group (P<0.05). Conclusion: High dose fructose could affect several parameters of the liver and pancreas of SD rats， and had potential damage to their functions. Low and medium dose fructose intake had no significant effect on the liver and pancreas functions of rats.