Objective: To explore the effect of Kaihua Longding green tea water extract on intervening AMPK signaling pathway of liver hepatic steatosis and gut microbiota dysbiosis in high-fat diet mice. Methods: Detect the dynamic changes trend of main functional components of samples from each processing procedures in Kaihua Longding green tea. After acclimation feeding for 1 week， 30 mice were randomly divided into the following three groups: normal control group (NC)， high-fat diet group (HF) and Longding green tea group (LD)， and were continuously administrated (NC and HF were given normal saline， LD was given tea water extract (300 mg/kg bw) for 8 weeks. The changes of body weight， Lee's index， blood glucose levels， serum lipid， pathobiology in liver tissues， AMPK signaling pathway in liver and gut microbiota were measured. Results: During processing procedures， the main functional components of Kaihua Longding green tea undergone certain changes. Compared to the "Fresh leaves" sample， the contents of tea polyphenol， free amino acids， caffeine， and moisture in the "Drying" sample were respectively reduced by 7.90%， 6.20%， 0.47%， and 71.05 g/100 g， while the contents of soluble sugar and water extract were respectively increased by 0.01% and 0.10%. Compared to the HF group， the LD group exhibited a significant difference (P < 0.05) in body weight (26.67 g)， Lee's index (3.13)， area under the glucose curve (1 505.25 mmol·min/L)， and serum biochemical indices such as total cholesterol (TC) (4.23 mmol/L)， and total triglycerides (TG) (0.76 mmol/L)， and also reduced the level of AMPK signaling pathway related genes and proteins of liver tissue in mice (P < 0.05). The results of gut microbiota analysis showed that Kaihua Longding green tea water extract decreased the ratio of Firmicutes/Bacteroidetes (F/B)， and increased the growth of beneficial bacteria， including Rikenellaceae， Blautia and Alistipes. Conclusion: The effect of Kaihua Longding green tea water extract on intervening AMPK signaling pathway of liver hepatic steatosis and gut microbiota dysbiosis in high-fat diet mice was effective.