Probiotic and Enzymatic Characteristics of β-glucosidase Producing Lactobacillus plantarum Isolated from Pickled Vegetables
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(1.College of Food Science and Engineering, Bohai University, Jinzhou 121013, Liaoning;2.Hangzhou Xiaoshan Agriculture Development Co., Ltd., Hangzhou 311215)

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    Abstract:

    Lactic acid bacteria play an essential role in fermentation due to their characteristics and secretion of various metabolites. In this study, two lactic acid bacteria with high β-glucosidase activities were screened from kimchi and identified as Lactobacillus plantarum PC7-C6 and PC7-C11). The probiotic characteristics of the two strains and the enzymatic characteristics of PC7-C11which has higher enzyme production ability, were analyzed. The results showed that the two strains were γ-hemolysis, sensitive to ampicillin and clarithromycin, moderately sensitive to tetracycline, erythromycin, and clindamycin, and could grew at pH 4. The auto-aggregation rate of the two isolates ranged from 50% to 64%, and the co-aggregation rates with Escherichia coli were more than 60% after co-incubating for 8 hours. The hydrophobicity of the isolates towards ethyl acetate was 70.41% and 61.19%, and the hydrophobicity of the isolates towards xylene and n-hexane was between 30% and 40%. The two isolates could inhibit the growth Escherichia coli, Hafnia alvei, Staphylococcus saprophyticus, Serratia marcescens, and Staphylococcus equorum. The antioxidant capacity of PC7-C11 was superior to that of PC7-C6, with a 59.33% scavenging rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 53.49% scavenging rate of hydroxyl radicals respectively. The enzymatic characteristics of PC7-C11 showed that the optimal reaction temperature and pH for enzyme activity were 40 ℃ and pH 7. Ethanol, glucose, dimethyl sulfoxide, SDS, EDTA, Fe2+, Mg2+, Na+, and Ca2+ inhibited enzyme activity to varying degrees, while K+ has a certain promoting effect on enzyme activity.

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  • Received:September 12,2023
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  • Online: October 21,2024
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