Graptopetalum paraguayense is an edible succulent with multiple physiological properties. In this study， hyperuricemia (HUA) mouse model was established using hyperuricemia diet (containing 20% yeast extract and 4% potassium oxyazinate)， and the effects of Graptopetalum paraguayense juice/ crude polysaccharide on serum uric acid and gut microbiota in HUA mice were investigated. The results showed that the serum uric acid content of HUA mice was decreased significantly (P<0.05)， as evidenced by 14.76% and 29.83% reduction after Graptopetalum paraguayense juice/crude polysaccharide treatment. Moreover， the blood urea nitrogen content of HUA mice was also decreased 22.56% and 18.05%， respectively. Additionally， Graptopetalum paraguayense juice/ crude polysaccharide could also attenuate the glomerulus tumidness， renal tubule epithelial cell atrophy and inflammatory cell infiltration of HUA mice； it also inhibited the expression of URAT1 and GLUT9， promoted the expression of ABCG2， thereby promoting the excretion of uric acid. Moreover， Graptopetalum paraguayense juice/crude polysaccharide decreased the abundances of Desulfovibrionaceae unclassified and Desulfovibrio， and increased the abundances of beneficial bacteria including Muribaculum， Alloprevotella and Bacteroides. These results of our study are conducive to the development and utilization of Graptopetalum paraguayense， and also can provide theoretical reference for the development of Graptopetalum paraguayense related uric acid lowering functional food.