Purpose: To explore the effect of soybean hull polysaccharides on the growth and adhesion of probiotics. Methods: The absorbance of OD600nm， growth time and pH value were explored to analyze the proliferation capacity of Lactobacillus paracasei 6244. A mouse intestinal probiotic adhesion model was established to study the effects of soybean hull polysaccharides on the adhesion capacity of Lactobacillus paracasei 6244 from measuring the changes of auto-aggregation， co-aggregation， hydrophobicity and intestinal microenvironment. The results showed that 0.5 mg/mL C-ASP and HW-ASP significantly accelerated the growth rate of Lactobacillus paracasei 6244， shortened the growth time to 4.08 h and 4.69 h， respectively， but had no significant effect on the acid-producing capacity of Lactobacillus paracei 62445. In addition， C-ASP can significantly improve the surface hydrophobicity and co-aggregation of Lactobacillus paracasei 6244， promoted the secretion of exopolysaccharides， surface proteins and other adhesins and intestinal mucus， and increased the colonization of Lactobacillus paracasei 6244 in different intestinal tissue， especially in the jejunum and ileum segments， the adhesion rate of Lactobacillus paracasei 6244 increased by more than 32%. Conclusion: Soybean hull polysaccharide can promote the proliferation and adhesion of intestinal probiotics， which is conducive to the active role of intestinal probiotics and the improvement of intestinal microenvironment.