Pseudomonas and Aeromonas are the dominant spoilage bacteria in aquatic products， and their spoilage characteristics are regulated by quorum sensing system mediated by N-acyl homoserine lactone signaling molecules. In this study， salmon infected by Pseudomonas fluorescein alone or mixed with Aeromonas hydrophila were used as a model. The aerobic plate count (APC)， pH， water holding capacity (WHC)， thiobarbituric acid (TBA)， total volatatile basic nitrogen (TVB-N) and other indexes were used to evaluate the fresh-preserving effect of crude cell extract (CCE) from Bacillus cereus YF-2 with quorum quenching activity on refrigerated salmon. The results showed that the growth of P. fluorescens and A. hydrophila was not affected when the protein concentration of CCE from B. cereus YF-2 was 10-160 μg/mL. Treatment with 160 μg/mL of B. cereus YF-2 CCE could slow down the rise rate of the APC， pH， TBA and TVB-N， as well as the decline rate of WHC. Compared with salmon samples infected with P. fluorescens alone or mixed with A. hydrophila， adding B. cereus YF-2 CCE could prolong the storage period of salmon for 2 days and 4 days， respectively. This study was expected to provide a theoretical basis for the application of quorum quenching bacteria to control the spoilage of aquatic products and the development of new biological preservatives.