Analysis of Bacterial Flora Structure and Flavor Quality of Spontaneous Fermentation Swiss Chard
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(1.Department of Food Science and Engineering, Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Provincial Key Laboratory of Food Microbiology and Nutritional Health, Ningbo University, Ningbo 315832, Zhejiang;2.Key Laboratory of Detection and Risk Prevention of Key Hazardous Materials in Food, China General Chamber of Commerce, Ningbo Key Laboratory of Detection, Control, and Early Warning of Key Hazardous Materials in Food, Ningbo Academy of Product and Food Quality Inspection (Ningbo Fibre Inspection Institute), Ningbo 315048, Zhejiang;3.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095)

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    Abstract:

    In order to explore the complex microbial system and volatile flavor components in the traditional fermentation process of Sichuan Swiss Chard, high-throughput sequencing and bioinformatics related technologies were used to comprehensively analyze the microbial flora structure of the Swiss Chard and its relation among the microbial species. The types and contents of organic acids in the Swiss Chard were determined by liquid chromatography. At the same time, headspace solid phase microextraction (HS-SPME) and gas chromatography-time of flight mass spectrometry (GC-TOFMS) were used to systematically analyze the volatile flavor components of Sichuan Swiss Chard samples. The results showed that the main contributors to the flora were Lactobacillus, unclassified-Cyanobacteria, Halomonas and Lentilactobacillus. The Swiss Chard contain six organic acids including tartaric acid, lactic acid, acetic acid and so on. And the highest content of lactic acid was 470.50 mg/100 mL. The relative content of acids in volatile flavor substances was the highest, mainly acetic acid, followed by alcohols such as 2-octanol and esters such as ethyl lactate. This study clarified the correlation between the microbial community structure and the formation of flavor substances in Sichuan fermented kimchi, aiming to provide a theoretical basis for further improving the traditional fermented kimchi process and large-scale production of inoculation control.

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  • Received:January 19,2024
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  • Online: March 05,2025
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