Exopolysaccharide produced by lactic acid bacteria (LAB) is the product of microorganism adapting to the environment， which has many beneficial effects such as inhibiting the growth of pathogenic bacteria， antioxidation， lowering blood sugar， and improving the taste， texture and flavor of fermented dairy products. In this study， Lacticaseibacillus paracasei(L. paracasei) ProSci-92， which was isolated from sour yak milk in Shangri-La Tibetan area， was used as the research object， and the whole genome of the strain was sequenced by Illumina Hiseq 4000 second-generation sequencing technology. The genome of L. paracasei ProSci-92 was 3 012 314 bp， encoding 2 975 genes. The results of gene annotation showed that L. paracasei ProSci-92 RAST a complete eps gene cluster. The length was 16 581 bp， including 19 genes related to extracellular exopolysaccharide (EPS) biosynthesis， such as wzb， LytR， wzg， epsC， epsD， glf1， glf2， glf3， wze， glf4， WelA and WelB. In addition， the results of real-time quantitative PCR (RT-qPCR) showed that almost all genes in eps gene cluster could be expressed， and the expression reached the maximum at 6 h. The smooth progress of this study provided help for the structural analysis of L. paracasei ProSci-92 EPS， and provided a theoretical basis for the industrial application of the strain.