To investigate the absorption characteristics of different types of dietary advanced lipid oxidation end products (ALEs) in the intestine and their effects on intestinal barrier integrity， this study employed a Caco-2 cell monolayer absorption model combined with high-resolution mass spectrometry to evaluate the transmembrane absorption patterns of bound ALEs. Using scanning electron microscopy and immunofluorescence analysis， the study further assessed the impact of dietary ALEs on intestinal barrier function and analyzed how intestinal barrier damage affects ALEs absorption characteristics. The results demonstrated that six types of bound ALEs， namely NPO， DHP-Lys， NP-peptide， DHP-peptide， NIAL and OMLI， can be absorbed in the intestine， primarily existing in dipeptide or tripeptide forms. Moreover， the presence of ALEs increased the apparent permeability coefficient of Caco-2 monolayers by 50.83%， decreased the transmembrane resistance by 53.28%， and led to reduced expression of tight junction proteins Occludin and ZO-1， as well as Mucin2， indicating that ALEs disrupted the integrity of the intestinal barrier. Further investigations revealed that under conditions of compromised intestinal barrier， the absorption states of ALEs change， particularly with increased absorption of DHP-peptide and NP-peptide in the intestine. In summary， ALEs can affect intestinal barrier function by disrupting the expression of tight junction proteins and mucins. When the intestinal barrier is impaired， the absorption characteristics of ALEs significantly alter， potentially affecting their distribution and metabolism in the body. These findings are crucial for understanding the behavior of ALEs in the intestine and their potential mechanisms of intestinal barrier damage， providing scientific evidence for preventing diet-related chronic diseases.