Objective: This study aimed to isolate and screen lactic acid bacteria with a high cholesterol degradation rate from Deang acid tea in Yunnan Province. The study also aimed to identify their physiological and biochemical characteristics， evaluate their safety and study their probiotics. Methods: Initially， a Lactobacillus with good characteristics was identified through screening for acid resistance， bile salt tolerance， acid production cholesterol-lowering ability and Intestinal colonization ability. After a series of identification and verification experiments， the strain was identified as Lactiplantibacillus plantarum and named HYY-S10. The safety and probiotic properties of HYY-S10 were evaluated， including hemolysis， antibiotic resistance， bacteriostatic ability and gastroenteric fluid tolerance. Results: HYY-S10 was found to be non-hemolytic and exhibited resistance to antibiotics. It demonstrated good tolerance to stomach and intestinal fluids and high intestinal colonization capacity， as well as high acid production ability and antibacterial effect on Escherichia coli compared to other strains isolated from sour tea. The bacteria entered the logarithmic growth phase at 4 hours， reaching an OD600 nm value of 1.836. Moreover， it displayed a remarkable cholesterol degradation rate of 91%. Conclusion: This study successfully identified excellent lactic acid bacteria with cholesterol-lowering properties. These findings offer a theoretical foundation for the development of tea-derived probiotics and functional microecological preparations. However， further exploration is required to understand the cholesterol-lowering mechanism of these strains.