Objective: To investigate the effects of methionine-restricted diet on liver injury in mice with alcoholic fatty liver. Methods: 30 male C57BL/6J mice were randomly divided into 3 groups: normal diet group (0.86% methionine， intragastric infusion of distilled water， n=10)， alcoholic fatty liver model group (0.86% methionine， intragastric infusion of 15 mg/kg body weight/d 56% ethanol， n=10)， methionine restriction intervention group (0.17% methionine， intragastric infusion of 15 mg/kg body weight/d 56% ethanol， n=10). After the 4 week experiment， liver tissue morphology and structure were observed， liver and plasma lipid levels， liver function indexes， liver and plasma redox status related indexes， and key gene expression levels of liver lipid synthesis and decomposition were detected. Results: Compared with the alcohol model group， the MR intervention group exhibited a 22.68% and 19.46% reduction in liver weight and index， respectively； hepatic TG and TC levels decreased by 29.65% and 10.40%， while plasma TG， TC， and LDL-C levels decreased by 45.93%， 10.15%， and 20.29%， respectively； liver tissues showed obviously alleviated cellular morphological damage and lipid infiltration； plasma AST， ALT， and TBIL levels decreased by 14.43%， 34.23%， and 17.71%， respectively， while TP levels increased by 12.33%； hepatic T-AOC， SOD， and CAT levels increased by 30.14%， 35.48%， and 19.57%， whereas MDA and ROS levels decreased by 21.50% and 15.99%， respectively； plasma T-AOC， SOD， and CAT levels increased by 26.80%， 12.21%， and 17.64%， while MDA and ROS levels decreased by 31.61% and 23.79%， respectively； hepatic alcohol dehydrogenase activity increased by 53.39%， while γ-glutamyl transferase activity decreased by 9.21%； the expression of key genes in hepatic lipogenesis (FAS， ACC1， SCD1， and SREBP1c) was significantly downregulated， whereas the expression of key genes in lipid catabolism (HSL， PPARα， CPT1， CYP7A1， and LPL) was significantly upregulated (P<0.05). Conclusion: MR intervention can improve the antioxidant capacity， alleviate liver injury， improve lipid metabolism and reduce liver fat deposition in mice with alcohol-induced liver injury， and has an effective effect on alcohol-induced liver injury.